A Transcriptome Shotgun Assembly (TSA) BLAST database is now available. The sequences were initially included in nt but now have been segregated into a separate database. The TSA database is available from the BLAST home page under Basic BLAST at the nucleotide, tblastn, and tblastx links. These sequences are not available in nt. TSA is an archive of computationally assembled mRNA sequences from primary data such as EST and raw sequence reads. See http://www.ncbi.nlm.nih.gov/genbank/TSA.html for details.
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